Measuring Influenza A Virus and Peptide Interaction Using Electrically Controllable DNA Nanolevers

Electrically controllable deoxyribonuclic acid (DNA) nanolevers are used to investigate the binding interaction between Influenza A/Aichi/2/1968 and peptide called “PeB”, which specifically binds viral surface protein hemagglutinin. PeB is immobilized on gold electrodes of a “switchSENSE” biochip by conjugation DNA-strands that hybridized complementary anchors. The surface-tethered DNA strand carries fluorophore while multivalent arrangement carrying up three peptides. kept upright (static) applying negative potential. Signal read-out for this static measurement mode change in fluorescence intensity due changes local environment dye upon binding. Measurements virus-peptide show virus material peptides remains bound throughout time. Immobilized viruses subsequently as ligands characterize oligovalent hemagglutinin, revealing rate constants interaction. Moreover, A subtypes compared their behavior. Overall, paper shows ability immobilize sensor surface, allows target virus-proteins native environment. method therefore applicable virus-receptor interactions.